Nylon Monofilament Mold Three-dimensional Microfluidic Chips for Size-exclusion Microchip Electrophoresis: Application to Specific Online Preconcentration of Proteins

We present a lithography-free procedure for fabricating intrinsically three-dimensional microchannels within PDMS elastomers using nylon monofilament molds. embedded monofilaments in an uncured composite to fabricate straight channels of desired length, use as molds form the microchannels. Next, we fabricated two layer devices consisting dialysis membranes, which preconcentrate specific proteins accordance with molecular weight, between layers substrates Because membrane isolation, analyte exchange fluidic can be precisely controlled by applied voltage. More importantly, given that only small molecules pass through membrane, integrated is suitable sieving or size exclusion concentrator prior microchip electrophoresis. Researchers our design online purification and preconcentration presence excess reagent immediately after fluorescent labeling. This method’s technical advantage microstructures, such have circular cross-section, are readily attainable straightforward manner without specialized equipment. Our method low-cost, environmentally sustainable microfluidic devices, will render processes more accessible easy implement.